2019-08-15 11:36:532025-01-16 15:20:54
The protein
[SW|Domains]
Exonuclease domain (aa 7-162) (according to UniProt)
[SW|Helicase ATP-binding domain] (aa 250-510) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 741-897) (according to UniProt)
[SW|Exonuclease domain] (aa 7-162) (according to UniProt)
[SW|Helicase ATP-binding domain] (aa 250-510) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 741-897) (according to UniProt)
Biological materials
Mutant
MGNA-A423 (dinG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/423 NBRP B. subtilis, Japan]
BKE22400 ([[gene|dinG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
BKK22400 ([[gene|dinG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
MGNA-A423 (dinG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/423 NBRP B. subtilis, Japan]
BKE22400 ([[gene|dinG]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
BKK22400 ([[gene|dinG]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22400 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC